f Laboratory Cultivation of Some Human Parasitic Amoebae
- By G. L. Robinson
- First Published Online: 01 August 1968, Microbiology 53: 69-79, doi: 10.1099/00221287-53-1-69
- Subject: Article
- Issue Published:
SUMMARY: Cultural conditions and supplementary substances necessary for the laboratory cultivation of some human parasitic amoebae were investigated by using a basal solution containing inorganic salts, citrate and lactate. Three supplementary components were found necessary: starch grains, animal protein (soluble or insoluble) and living bacteria. Restraint of bacterial growth by antibiotics, and of the development of an alkaline reaction (due to pellicle-forming aerobes) by carbon dioxide, improved the amoebic growth. Under these conditions, all the common parasitic amoebae examined, except Ioda-moeba butschlii, were grown and maintained for long periods in laboratory culture.
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